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. 2020 Mar 6;8(3):374. doi: 10.3390/microorganisms8030374

Table 2.

Mycobacteria detection and identification in positive small mammal specimens.

Rodent Species Mycobacterium Isolation MGIT PCR Result Direct PCR Result (Tissue Homogenate) Mycobacterium Identification Method Final Identification
A. sylvaticus Yes Positive (Mycobacterium sp.) Negative Reverse hybridization and ITS sequencing M. fortuitum
Apodemus sp. Yes Positive (Mycobacterium sp.) Negative Reverse hybridization M. intracellulare
A. sylvaticus Yes Positive (Mycobacterium sp.) Negative Reverse hybridization M. gordonae
A. sylvaticus Yes Positive (Mycobacterium sp.) Negative Reverse hybridization M. celatum
A. sylvaticus No Negative Positive (M. avium) IS900, ISMap02, IS1245 and IS901 Map
A. sylvaticus No Negative Positive (M. avium) IS900, ISMap02, IS1245 and IS901 Map
A. sylvaticus No Negative Positive (Mycobacterium sp.) ITS sequencing Mycobacterium sp.*

* ITS sequence with a percentage of identity of 82.91% with M. peregrinum IoA5 (BLAST).