Table 2. E. coli Protein Targeting in Different Subcellular Organelles of Host Cell (ER, ER Membranes, and Golgi Apparatus Membranes) of Host Cells with (%) Expected Accuracy, pI Value, Molecular Weight (dalton), and Functions.

accession no.	protein name	mol. weight (dalton)	pI value	protein targeting results using LocTree3	expected accuracy (%)	functions in bacteria
P0A9X4	rod shape-determining protein MreB	36 952.4	5.19	ER	80	involved in the formation of the rod shape of the cell. May act as a negative regulator of FtsI
P24232	Flavohemoprotein	43 867.66	5.48	ER	81	involved in nitric oxide (NO) detoxification in anaerobic processes, termed nitric oxide dioxygenase (NOD) reaction that utilizes O2 and NAD(P) H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Induced by NO (under aerobic conditions), nitrite, nitrate(under anaerobic conditions), nitroso compounds, and paraquat. Induced by low pH during anaerobic growth
P23869	peptidyl–prolyl cis–trans isomerase B	18 153.47	5.51	ER	84	see PpiA
P0AB77	2-amino-3-ketobutyrate coenzyme A ligase	43 117.04	5.64	ER membrane	83	negatively regulated by Lrp
P3687	hypothetical ABC transporter ATP-binding protein YadG	34 647.07	8.44	ER membrane	82	not available
P6075	lipid A export ATP-binding/permease protein MsbA	64 460.71	8.62	ER membrane	82	involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane
PO2942	methyl-accepting chemotaxis protein I	59 442.99	4.88	ER membrane	89	not available
P67910	ADP-l-glycero-d-manno-heptose-6-epimerase	34 893.17	4.8	Golgi apparatus membrane	89	catalyzes the interconversion between ADP-d-glycero-β-d-manno-heptose and ADP-l-glycero-β-d-manno-heptose via an epimerization at carbon 6 of the heptose. Completely inhibited by ADP and ADP-glucose and partially inhibited by ATP and NADH. It is induced by heat shock
P76372	chain length determinant protein	36 454.74	5.43	Golgi apparatus membrane	88	confers a modal distribution of chain length on the O-antigen component of lipopolysaccharide (LPS). Gives rise to a reduced number of short-chain molecules and increased numbers of longer molecules
P1587	quinoprotein glucose dehydrogenase	86 747.35	5.4	Golgi apparatus membrane	82	may be involved in energy conservation rather than in sugar metabolism