Table 3. E. coli Protein Targeting in Peroxisomes of Host Cell, Including Percentage (%) Expected Accuracy, pI Value, Molecular Weight (dalton), and Functions.

accession no.	protein name	mol. weight (dalton)	pI value	protein targeting results using LocTree3	expected accuracy (%)	functions in bacteria
P33232	l-lactate dehydrogenase	42 728.19	6.33	peroxisome	83	induced by l-lactate, aerobically
P0AET8	7α-hydroxysteroid dehydrogenase	26 778.59	5.22	peroxisome	83	7α-dehydroxylation of cholic acid, yielding deoxycholic acid and lithocholic acid, respectively. Highest affinity with taurochenodeoxycholic acid
P0A6F3	glycerol kinase	56 099.57	5.36	peroxisome	86	a key enzyme in the regulation of glycerol uptake and metabolism. The activity of this regulatory enzyme is affected by several metabolites. The inhibition by fructose 1,6-biphosphate causes alterations in the quaternary structure of the enzyme. Induced by l-α-glycerol-3-phosphate. Increases following exposure to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol
P0ABU0	naphthoate synthase	31 633.08	5.99	peroxisome	93	converts O-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoic acid (DHNA). Involved in menaquinone biosynthesis
P0ADG7	inosine-5′-monophosphate dehydrogenase	52 022.45	6.02	peroxisome	85	involved in the first step of GMP biosynthesis from IMP. Induced by low pH during anaerobic growth
P0ABH7	citrate synthase	48 014.99	6.21	peroxisome	87	involved in the tricarboxylic acid cycle and is allosterically inhibited by NADH
P0AD96	Leu/Ile/Val-binding protein	36 772.53	5.28	peroxisome	82	component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids. Increases following exposure to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol and in the physiological short-term adaptation to glucose-limitation
P04816	leucine-specific binding protein	36 982.71	5.07	peroxisome	82	component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids. Increases following exposure to the uncoupler of oxidative phosphorylation 2,4-dinitrophenol. It decreases after benzoic acid treatment
P65556	putative Nudix hydrolase YfcD	20 375.86	4.7	peroxisome	80	not available