Table 2.
CD spectrum analysis of conotoxin-Ac1, its variant and its mutants.
| No. | Name | α-helix (%) | β-sheet (%) | β-turn (%) | Random Coil (%) |
|---|---|---|---|---|---|
| 1 | conotoxin-Ac1 | 44.9 | 27.7 | 15 | 12.4 |
| 2 | conotoxin-Ac1-O6P | 51 | 21.9 | 16.1 | 11 |
| 3 | conotoxin-Ac1-N1A | 54.7 | 35 | 8.1 | 2.2 |
| 4 | conotoxin-Ac1-Y2A | 48.4 | 23.4 | 16.3 | 11.9 |
| 5 | conotoxin-Ac1-Y3A | 48.9 | 23 | 16.6 | 11.5 |
| 6 | conotoxin-Ac1-L4A | 49.6 | 25.7 | 13.2 | 11.5 |
| 7 | conotoxin-Ac1-Y5A | 8.1 | 46.8 | 27.3 | 17.8 |
| 8 | conotoxin-Ac1-O6A | 46.6 | 26.6 | 6.4 | 20.4 |
| 9 | conotoxin-Ac1-R8A | 54.4 | 23.5 | 14.1 | 8 |
| 10 | conotoxin-Ac1-O9A | 41.2 | 27.4 | 16.2 | 15.2 |
| 11 | conotoxin-Ac1-E10A | 37.1 | 30.2 | 16.1 | 16.6 |
| 12 | conotoxin-Ac1-N11A | 51.1 | 24.6 | 14.3 | 10 |
| 13 | conotoxin-Ac1-S12A | 46.7 | 25.9 | 16.8 | 10.6 |
| 14 | conotoxin-Ac1-W13A | 49.2 | 16.2 | 23.1 | 11.5 |
| 15 | conotoxin-Ac1-W14A | 45.9 | 23.1 | 16.6 | 14.4 |
| 16 | conotoxin-Ac1-T15A | 49 | 21.3 | 13.6 | 16.1 |
| 17 | conotoxin-Ac1-15 * | 48.7 | 26.7 | 12.7 | 11.9 |
| 18 | conotoxin-Ac1-E10γ | 50.7 | 23.1 | 9.4 | 16.8 |
| 19 | conotoxin-Ac1-E10γW14γ | 52.9 | 26.8 | 9.9 | 10.4 |
| 20 | conotoxin-Ac1-E10γW14γ15 * | 47.2 | 18.2 | 18.1 | 16.5 |
* C-terminal amidation; γ: γ-carboxyglutamic acid; O: hydroxyproline.