Table 6.
Analgesic activity of conotoxin-Ac1 and conotoxin-Ac1-O6P, as determined by the hot-plate test ( ± s).
| No. | Group | Pain Threshold (s) | ||||
|---|---|---|---|---|---|---|
| 15 min | 30 min | 60 min | 120 min | 180 min | ||
| control | 15.39 ± 5.03 | 13.21 ± 4.56 | 15.14 ± 4.63 | 12.71 ± 4.64 | 21.91 ± 4.93 | |
| morpholine 1mg/kg | 17.19 ± 2.22 | 41.80 ± 4.58 | 50.41 ± 6.59 | 50.82± 4.35 | 47.11 ± 4.04 | |
| 1 | conotoxin-Ac1 | 16.17 ± 2.49 | 23.16 ± 3.80 | 34.18 ± 2.35 | 37.19 ± 7.31 | 33.51 ± 4.66 |
| 2 | conotoxin-Ac1-O6P | 15.84 ± 1.76 | 23.19 ± 5.92 | 38.90 ± 10.09 | 38.47 ± 6.59 | 33.44 ± 1.72 |
| 3 | conotoxin-Ac1-N1A | 17.33 ± 3.77 | 21.34 ± 7.15 | 34.71 ± 6.91 | 32.13 ± 9.19 | 28.26 ± 9.14 |
| 4 | conotoxin-Ac1-Y2A | 16.81 ± 1.56 | 22.28 ± 3.40 | 36.77 ± 8.12 | 33.84 ± 7.51 | 29.90 ± 7.51 |
| 5 | conotoxin-Ac1-Y3A | 17.56 ± 2.49 | 23.60 ± 8.28 | 35.95 ± 7.67 | 32.14 ± 6.87 | 28.77 ± 6.32 |
| 6 | conotoxin-Ac1-L4A | 17.70 ± 2.71 | 23.05 ± 7.12 | 34.09 ± 6.75 | 37.28 ± 5.09 | 34.44 ± 4.78 |
| 7 | conotoxin-Ac1-Y5A | 16.45 ± 4.79 | 21.88 ± 11.18 | 31.76 ± 9.33 | 32.09 ± 6.26 | 30.40 ± 6.01 |
| 8 | conotoxin-Ac1-O6A | 16.12 ± 2.49 | 22.39 ± 10.40 | 31.53 ± 7.59 | 34.82 ± 8.00 | 32.03 ± 8.56 |
| 9 | conotoxin-Ac1-R8A | 16.93 ± 3.83 | 20.24 ± 5.39 | 31.80 ± 10.83 | 35.93 ± 7.64 | 31.89 ± 7.64 |
| 10 | conotoxin-Ac1-O9A | 16.75 ± 3.65 | 25.45 ± 5.02 | 29.52 ± 6.69 | 31.83 ± 8.39 | 29.70 ± 7.97 |
| 11 | conotoxin-Ac1-E10A | 16.69 ± 3.12 | 19.84 ± 8.99 | 28.05 ± 5.57 # | 36.62±8.41 | 31.61 ± 8.41 |
| 12 | conotoxin-Ac1-N11A | 16.67 ± 0.94 | 25.32 ± 9.81 | 35.82 ± 7.51 | 34.24 ± 7.90 | 32.40 ± 7.90 |
| 13 | conotoxin-Ac1-S12A | 15.73 ± 3.43 | 25.41 ± 7.77 | 35.24 ± 6.58 | 35.20 ± 8.66 | 31.50±7.95 |
| 14 | conotoxin-Ac1-W13A | 16.48 ± 2.48 | 20.78 ± 6.92 | 30.26 ± 10.33 | 35.83 ± 7.00 | 32.21 ± 6.44 |
| 15 | conotoxin-Ac1-W14A | 15.96 ± 3.60 | 21.64 ± 8.98 | 28.39 ± 5.13 | 36.42 ± 7.12 | 31.73 ± 6.40 |
| 16 | conotoxin-Ac1-T15A | 17.78 ± 2.69 | 19.56 ± 8.59 | 27.19 ± 7.42 # | 30.83 ± 7.13 | 26.62 ± 6.40 |
| 17 | conotoxin-Ac1-15 * | 16.06 ± 3.31 | 30.26 ± 7.69 | 39.59 ± 9.63 | 41.93 ± 10.04 | 38.03 ± 9.11 |
| 18 | conotoxin-Ac1-E10γ | 16.35 ± 3.19 | 26.50 ± 7.79 | 38.44 ± 8.80 | 44.33 ± 4.91 ## | 36.17 ± 8.28 |
| 19 | conotoxin-Ac1-E10γW14γ | 17.90 ± 2.84 | 33.94 ± 7.88 # | 39.64 ± 5.44 # | 47.61 ± 2.26 # | 44.46 ± 2.11 ## |
| 20 | conotoxin-Ac1- E10γW14γ15 * | 15.98 ± 3.55 | 33.23 ± 3.01 # | 51.08 ± 3.52 ## | 54.24 ± 3.09 ## | 50.55 ± 2.88 ## |
* C-terminal amidation; γ: γ-carboxyglutamic acid; O: hydroxyproline; # p < 0.05 vs. the control group; ## p < 0.01 vs. the control group.