Table 1.
Reference samples used in this study and threshold cycle (Ct) results.
| Sample Code | Species | Common Name (FAO) | Geographic Origin | Ct Mean ± SD |
|---|---|---|---|---|
| SOFF2 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Vigo | 12.98 ± 0.32 |
| SOFF3 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Cambados | 14.05 ± 0.26 |
| SOFF4 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Cambados | 14.80 ± 0.04 |
| SOFF5 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Cambados | 16.16 ± 0.35 |
| SOFF6 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Vigo | 13.57 ± 0.70 |
| SOFF7 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Vigo | 15.37 ± 0.10 |
| SOFF8 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Vigo | 13.51 ± 0.07 |
| SOFF9 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27) | 14.11 ± 0.20 |
| SOFF10 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27) | 15.65 ± 0.21 |
| SOFF11 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27) | 13.86 ± 0.48 |
| SOFF12 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27) | 13.25 ± 0.39 |
| SOFF15 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Algarve | 12.72 ± 0.06 |
| SOFF16 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Algarve | 12.59 ± 0.19 |
| SOFF17 | Sepia officinalis | Common cuttlefish | Atlantic, Northeast (FAO 27.9) Algarve | 13.98 ± 0.12 |
| SBER 2 | Sepia betheloti | African cuttlefish | Atlantic, Eastern Central (FAO 34) | ≥40 |
| SBER 3 | Sepia betheloti | African cuttlefish | Atlantic, Eastern Central (FAO 34) | ≥40 |
| SORB 4 | Sepia orbygniana | Pink cuttlefish | Atlantic, Northeast (FAO 27) | ≥40 |
| SORB 5 | Sepia orbygniana | Pink cuttlefish | Atlantic, Northeast (FAO 27) | ≥40 |
| SPHA 1 | Sepia pharaonis | Pharaon cuttlefish | Indian Ocean, Western (FAO 51) | ≥40 |
| LVUL 2 | Loligo vulgaris | European squid | Western Central Atlantic (FAO 31) | 27.00 ± 0.16 |
| LVUL 1 | Loligo vulgaris | European squid | Atlantic, Northeast (FAO 27) | 26.03 ± 0.28 |
| LVUL 5 | Loligo vulgaris | European squid | Atlantic, Northeast (FAO 27) | 29.15 ± 0.14 |
| LVUL 3 | Loligo vulgaris | European squid | Western Central Atlantic (FAO 31) | ≥40 |
| LVUL 4 | Loligo vulgaris | European squid | Western Central Atlantic (FAO 31) | ≥40 |
| LVUL 6 | Loligo vulgaris | European squid | Western Central Atlantic (FAO 31) | ≥40 |
| LVUL 7 | Loligo vulgaris | European squid | Western Central Atlantic (FAO 31) | ≥40 |
| LVUL 8 | Loligo vulgaris | European squid | Western Central Atlantic (FAO 31) | 29.80 ± 0.40 |
| LREY 1 | Loligo reynaudi | Cape Hope squid | Atlantic, Southeast (FAO 47) | ≥40 |
| IILL 2 | Illex illecebrosus | Northern Shortfin squid | Atlantic, Northwest (FAO 21) | ≥40 |
| TEBL 1 | Todaropsis eblanae | Lesser flying squid | Atlantic, Northeast (FAO 27) | ≥40 |
| TPAC 3 | Todarodes pacificus | Japanese flying squid | Pacific, Northwest (FAO 61) | ≥40 |
| ICOI 10 | Illex coindetii | Southern shortfin squid | Atlantic, Northeast (FAO 27) | ≥40 |
| LGAH 9 | Loligo gahi | Patagonian squid | Pacific, Southeast (FAO 87) | ≥40 |
| MHYA 8 | Martialia hyadesi | Sevenstar flying squid | Atlantic, Antarctic (FAO 48) | ≥40 |
| NSLO6 | Nototodarus sloanii | Wellington flying squid | Pacific, Southwest (FAO 81) | ≥40 |
| TSAG 1 | Todarodes sagittatus | European flying squid | Atlantic, Northeast (FAO 27) | ≥40 |
| OVUL 142 | Octopus vulgaris | Common octopus | Atlantic, Northeast (FAO 27) | ≥40 |
| OCYA 3 | Octopus cyanea | Big blue octopus | Pacific, Western Central (FAO 71) | ≥40 |
| OCYA 4 | Octopus cyanea | Big blue octopus | Pacific, Western Central (FAO 71) | ≥40 |
| OMIM 1 | Octopus mimus | Changos octopus | Pacific, Southeast (FAO 87) | ≥40 |
| ECIR 143 | Eledone cirrhosa | Horned octopus | Atlantic, Northeast (FAO 27) | ≥40 |
| DGIG 1 | Dosidicus gigas | Jumbo squid | Pacific, Southeast (FAO 87) | ≥40 |
| AMEM 1 | Amphioctopus membranaceus | Webfoot octopus | Indian Ocean, Western (FAO 51) | ≥40 |
FAO: Food and Agriculture Organization. SD: Standard Deviation.