Table 1.

Accuracy, limit of detection (LOD) and limit of quantification (LOQ) results of TCA cycle intermediates.

Analyte	% Mean Accuracy a at Different Standard Concentration Level											LoQ (ng/mL)	LoD (ng/mL)
	L-1	L-2	L-3	L-4	L-5	L-6	L-7	L-8	L-9	L-10	L-11
cis-Aconitic acid	85.12	80.24	86.54	81.91	94.99	85.73	99.17	104.58	101.65	99.12	100.07	11.72	8.79
Citric acid	103.38	86.48	90.49	93.10	95.52	104.64	101.99	103.79	103.69	101.25	98.51	122.07	12.21
Fumaric acid	96.42	115.90	97.13	109.16	97.60	101.16	99.09	95.49	95.91	98.73	102.65	23.44	17.58
Isocitric acid	102.28	106.47	135.45	138.34	116.83	103.05	91.41	83.08	86.35	95.54	114.94	29.30	14.65
Malic acid	101.33	86.40	82.40	83.07	101.73	104.68	103.53	105.99	104.21	102.07	97.29	6.25	3.13
Pyruvic acid	98.50	86.14	118.65	108.54	100.45	105.05	99.12	96.39	86.12	81.99	86.57	244.14	183.11
Oxoglutaric acid	99.61	101.24	125.54	108.43	99.51	101.72	99.24	98.11	98.44	98.38	98.45	122.07	61.04
Glycine	104.04	116.45	90.41	86.32	116.92	104.29	101.37	101.97	101.40	102.70	98.06	122.07	61.04
l-Alanine	94.24	109.23	101.87	101.99	105.04	103.66	102.49	99.33	97.90	92.59	91.66	122.07	12.21
l-Serine	97.38	100.70	117.07	107.11	100.20	101.90	99.76	100.26	99.10	91.96	96.08	29.30	2.93
l-Aspartic acid	97.84	95.56	121.37	102.72	101.81	101.74	102.04	102.50	99.70	89.49	92.91	11.72	5.86
l-Glutamine	98.28	99.04	104.86	104.54	104.57	105.26	101.34	99.12	89.24	75.12	63.02	122.07	6.10
l-Glutamic acid	97.16	88.41	108.66	102.11	102.84	96.74	97.30	99.39	100.14	100.13	98.48	29.30	2.93
l-Lactic acid	95.19	109.72	103.53	93.83	97.68	99.92	99.00	99.98	101.23	99.01	100.90	527.34	52.73
Succinic acid	98.98	101.25	104.02	95.32	98.26	101.43	100.23	103.12	102.37	98.57	96.42	23.44	11.72
d-Glucose	96.08	103.03	106.53	104.76	100.91	103.12	102.59	101.29	98.12	94.97	88.60	976.56	97.66

^a: % mean accuracy is mean of accuracy of three calibration standard.