. 2020 Mar 12;10(3):103. doi: 10.3390/metabo10030103

Table 2.

Results of recovery, intra- and inter-day precision of TCA cycle intermediates in Serum and Tissue.

Analyte	Linearity Regression r² Value ^a	Intra-Day Precision of High, Medium and Low Concentrations, % RSD			Inter-Day Precision of High, Medium and Low Concentrations, % RSD			Mean Recovery Serum (%) ^b	Mean Recovery Tissue (%) ^b	Mean Recovery Cell (%) ^b
Analyte	Linearity Regression r² Value ^a	HQC	MQC	LQC	HQC	MQC	LQC	Mean Recovery Serum (%) ^b	Mean Recovery Tissue (%) ^b	Mean Recovery Cell (%) ^b
cis-Aconitic acid	0.999	3.83	12.10	14.90	2.73	11.30	15.90	89.30	223.82	107.24
Citric acid	0.993	2.69	5.79	20.24	6.24	5.81	19.84	100.59	125.73	104.36
Fumaric acid	0.995	2.10	5.18	11.63	2.60	5.74	19.34	89.99	77.24	102.54
Isocitric acid	0.990	6.89	11.31	19.57	6.19	17.27	19.82	90.53	111.40	87.04
Malic acid	0.992	1.46	8.61	21.87	1.66	7.56	19.23	104.28	105.66	92.01
Pyruvic acid	0.990	3.36	4.06	14.51	10.86	8.91	12.06	85.00	132.64	91.48
Oxoglutaric acid	0.998	2.17	4.87	11.88	2.66	4.33	11.72	79.93	108.26	86.96
Glycine	0.996	3.20	4.84	17.26	3.95	6.59	19.06	105.34	111.00	108.73
l-Alanine	0.994	2.52	2.68	12.20	2.29	3.54	13.76	106.21	104.72	102.91
l-Serine	0.994	1.95	4.95	11.27	2.58	4.43	16.25	113.68	139.08	104.54
l-Aspartic acid	0.994	6.82	3.27	11.28	5.71	5.13	12.07	107.72	100.49	105.43
l-Glutamine	0.994	3.96	3.55	8.09	4.99	3.38	7.64	113.09	111.81	105.14
l-Glutamic acid	0.996	1.91	2.93	26.47	3.69	5.63	18.47	118.35	145.97	109.48
l-Lactic acid	0.992	1.34	4.93	18.97	1.43	4.30	17.93	103.81	79.26	107.18
Succinic acid	0.997	2.51	4.28	19.51	2.20	4.59	19.92	100.68	151.24	97.04
d-Glucose	0.996	1.92	2.72	3.86	2.25	2.69	3.74	96.36	101.98	100.45

^a: Lowest r² value across three calibration sets during validation; ^b: Mean recovery of three QC levels.