Table 1.

Summary of Position Effects on Gene Expression Characterized in S. cerevisiae.

Construct/Analysis	Position Effect Observed	Ref.
pTEF1-LacZ and pACT1-LacZ	8-fold difference in expression across 20 integration sites.	[63]
pPRS3-LacZ	14-fold difference in expression across 18 integration sites.	[64]
pTEF-KANR	4-fold variation in expression across chromosome I.	[62]
pTEF-KANR in yeast knock out collect	Characterized the ‘Neighboring Gene Effect’. Transcription disruption by the KANR cassette resulted in missannotation of gene function in genetic screens (parenthesis represents frequence of missannotation): telomere length mutant screen (24.1%), resoponse to rapamycin screen (21.5%), topoisomerase mutant sensitivity (15.7%), and 5-fluorouracil sensitivity (7.2%).	[58]
Double deletion mutant screen	‘Neighboring Gene Effect’ resulted in 18% global missannotation of a systematic synthetic double deletion screen.	[59]
pRPL5-GFP	Characterized effects from 63 loci on chromosome I and 482 total sites globally. 22.6% of integration sites exhibited significant expressional differences, 36.5% demonstrated significant transcriptional noise, and there was 15-fold deviation in overal expression levels.	[60]
pTEF1-RFP and pURA3-RFP	13-fold difference in expression across 1044 integration sites. Different promoters resulted in different overall levels of expression; however, position affected both constructs to the same degree.	[61]