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. 2020 Apr 1;36(2):157–169. doi: 10.5423/PPJ.OA.01.2020.0004

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© The Korean Society of Plant Pathology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — Effect of nitrogen sources used to culture lactic acid bacteria (LAB) on the reduction of soft rot caused by Pectobacterium carotovorum. (A) The suppressive effectiveness of J02 (pH 4.1) and J13 (pH 5.3) fermented in the modified De Man, Rogosa and Sharpe (MRS) medium containing dextrose as the carbon source and different nitrogen sources on the reduction of soft rot severity on radish. LAB strains were grown in the modified MRS, in which peptone and beef extract were replaced with 2% each of the nitrogen sources as indicated for 3 days. After adding 1% chitosan, the resultant solution was tested for the reduction of soft rot on radish using a filter paper disc assay. Means followed by the same case letters in each panel were not significantly different (P ≤ 0.05). (B) The effect of surimi in a combination with either glycerol or sucrose on the reduction of soft rot severity on radish. J02 was cultured in the modified MRS containing different concentrations of surimi and 2% glycerol as the main nitrogen and carbon sources, respectively. J13 was cultured in the modified MRS with different concentrations of surimi and 2% sucrose.