Figure 7.

Effect of meroterpenes (AMTs) 1-8 on the activation of c-JUN N-terminal kinase (JNK) by measuring expression levels of the phosphorylated form (p-JNK) in HT-29 cells. The cells were treated for 24 h with the compounds (10 and 20 μg/mL for 1, 2, 5, 6, 7, and 8; 30 and 60 μg/mL for 3 and 4). The levels of p-JNK were measured by Western blot analysis and quantified with Image J analysis software. The result of control groups was set as 100% activity and test groups were compared to this value. The data shown are the means ± SE of three independent experiments. Significant differences from control group: * p < 0.05, ** p < 0.01, and *** p < 0.001.