Table 1.
Primer sequences used in quantitative real-time PCR assays. Keap1, kelch-like ECH-associated protein 1; CAT, catalase; GST, glutathione S-transferase; SOD, superoxide dismutase; HO-1, heme oxygenase-1; NQO1, NAD (P)H dehydrogenase, quinone 1; GCLC, glutamate-cysteine ligase catalytic subunit; GCLM, glutamate-cysteine ligase modifier subunit and CGT1, gamma-glutamyltransferase 1.
| Gene | Accession No. | Sequence (5′-3′) | Size (bp) |
|---|---|---|---|
| GAPDH | NM_001206359.1 | F-CGTCCCTGAGACACGATGGT | 194 |
| R-GCCTTGACTGTGCCGTGGAAT | |||
| Keap1 | XM_021076667.1 | F: CGTGGAGACAGAAACGTGGA | 239 |
| R: CAATCTGCTTCCGACAGGGT | |||
| Nrf2 | NM_001114671.1 | F: GACAAACCGCCTCAACTCAG | 183 |
| R: GTCTCCACGTCGTAGCGTTC | |||
| CAT | XM_021081498.1 | F:AGCTTTGCCCTTGCACAAAC | 119 |
| R:ACATCCTGAACAAGAAGGGGC | |||
| GST | NM_214389.2 | F:TTCAATGGCCGAGGCAGAAT | 216 |
| R: GAGGTTGTACTTGGTGGCGA | |||
| SOD | NM_001190422.1 | F: CATTCCATCATTGGCCGCAC | 118 |
| R: TTACACCACAGGCCAAACGA | |||
| HO-1 | NM_001004027.1 | F: CAAGCAGAAAATCCTCGAAG | 241 |
| R: GCTGAGTGTCAGGACCCATC | |||
| NQO1 | NM_001159613.1 | F: GATCATACTGGCCCACTCCG | 200 |
| R: GAGCAGTCTCGGCAGGATAC | |||
| GCLC | XM_003482164.4 | F:GGCGACGAGGTGGAATACAT | 123 |
| R: GTTTGGGTTTGTCCTTTCCCC | |||
| GCLM | XM_001926378.4 | F: GCATCTACAGCCTTACTGGGA | 180 |
| R: GTTAAATCGGGCGGCATCAC | |||
| GGT1 | NM_214030.1 | F:ATCACACCAGGAAAACAGCCA | 118 |
| R: CGGTAGATGTGGTGATCTGTGT |