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. 2020 Mar 20;8(3):436. doi: 10.3390/microorganisms8030436

Figure 1.

Figure 1

Analysis of glycosylated recombinant cholera toxin B subunit (rCTB) and CTB-OPSBa expressed in YeO9_52212. (A) YeO9_52212 was transformed with pET-CTB4573H or pET-pglL-CTB4573H and then induced with IPTG. A wild type strain was treated in the same way (negative control). Coomassie Blue staining (left) and western blotting (right) were performed. (B) The CTB-OPSBa (C-OPSBa) glycoprotein was purified from strain YeO9_52212 co-expressing PglL and rCTB. Samples were separated by 12% SDS-PAGE and analyzed by Coomassie Blue staining or western blotting using anti-His, anti-B. abortus (anti-Ba) or anti-Y. enterocolitica O:9 (anti-Ye) antibodies. (C) Coomassie Blue staining after native gel electrophoresis of C-OPSBa.