Figure 2.

Detection of CyHV-2 DNA and gene transcription in the moribund fish from temperature stress: (A) Quantification of the CyHV-2 DNA from three moribund fish suffered temperature stress by qPCR. White (1), gray (2), and black (3) bars respectively represent the different moribund fish. CyHV-2 DNA copies were calculated similarly, as described in Figure 1. The DNA copies shown are the mean of triplicates ± SD. (B) RT-PCR analysis of total RNA harvested from moribund fish in the presence (RT+) or absence (RT-) of reverse transcriptase: The amplicons of DNA pol (362 bp) and ORF99 (513 bp) are indicated. Lanes 1 to 6 respectively represent the tissue of the head, kidney, gills, midbrain, medulla oblongata, heart, and spleen, which were sampled from the same fish. The amplicons of β-actin gene are used as an internal control to ensure that comparable levels of input RNA were used in RT-PCR. One representative fish of three is shown.