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. 2020 Mar 21;8(3):445. doi: 10.3390/microorganisms8030445

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Examination of CyHV-2 DNA and gene transcription in the GCBlat1 cell line: (A) PCR analysis of the CyHV-2 DNA from GCBlat1 cells at indicated passages. Amplicons of DNA pol (362 bp) and ORF 99 (513 bp) are indicated. (B) qPCR analysis of the CyHV-2 DNA in GCBlat1 cells at indicated passages: CyHV-2 DNA copies were calculated similarly as described above. The data shown are the mean of triplicates ± SD. (C) RT-PCR analysis of total RNA harvested from GCBlat1 cells of passage 32: Lane 1, β-actin; lane 2, DNA pol (362 bp); lane 3, ORF99 (513 bp); M, DNA ladder. One representative experiment of three is shown.