Figure 5.

Examination of CyHV-2 DNA and gene transcription in GCBlat1 cells upon chemicals treatment: Cell viabilities of GCBlat1 cells were measured by an MTT assay following treatments with trichostatin A (TSA) (A) or phorbol 12-myristate 13-acetate (TPA) (B) (80, 40, 20, 10, and 5 μM) or dimethyl sulfoxide (DMSO) for 48 h. (C) qPCR analysis of the CyHV-2 DNA from GCBlat1 cells with TSA, TPA, DMSO, or without treatment (nc). CyHV-2 DNA copies were calculated similarly as described above. Data presented as the mean ± SD (n = 3). (D) RT-PCR analysis of total RNA harvested from treated and untreated GCBlat1 cells: The amplicons of DNA pol (362 bp) and ORF99 (513 bp) are indicated. The amplicons of β-actin gene are used as an internal control to ensure that comparable levels of input RNA were used in RT-PCR. M: DNA ladder; NC: negative control for PCR; lane 1: mock-treated GCBlat1 cells; lane 2: DMSO-treated GCBlat1 cells; lane 3: TPA-treated GCBlat1 cells; lane 4: TSA-treated GCBlat1 cells. One representative experiment of three is shown here. * p < 0.05