Table 1.

Primer pairs used to detect CyHV-2 DNA and gene transcription.

Namea	Target	Prime Sequence
DNA pol-F	DNA polymerase	5′-CCCAGCAACATGTGCGACGG-3′
DNA pol-R	DNA polymerase	5′-CCGTARTGAGAGTTGGCGCA-3′
ORF99-F	ORF99	5′-GTTACCCGAAGATACCCAT-3′
ORF99-R	ORF99	5′-TGCCGTTAGAAGGAGAAG-3′
actin-F	β-actin	5′-GCTATGTGGCTCTTGACTTCGA-3′
actin-R	β-actin	5′-CCGTCAGGCAGCTCATAGCT-3′
qpol-F	DNA polymerase	5′-GAAGGGCGGTAAAGTGTT-3′
qpol-R	DNA polymerase	5′-TCGCTGTGCGGGTATT-3′

^a Forward and reverse primers are indicated by F and R, respectively, in the primer names.