Table 3.

Repeatability and reproducibility of the real-time qPCR assays.

Coefficients of Variation Intra and Inter Assay for qPCR Assays
	28S Kinetoplastida spp.		28S Leish/Trypano pp.		5.8S Trypanosoma spp.
T. Congolense Load	Intra Assay	Inter Assay	Intra Assay	Inter Assay	Intra Assay	Inter Assay
1.06 × 106	1.61	2.84	5.13	1.10	1.87	4.43
1.06 × 105	1.88	2.15	4.24	3.04	5.28	7.71
1.06 × 104	2.90	1.82	1.70	0.37	3.44	7.60
1.06 × 103	1.78	9.25	2.72	3.40	2.63	8.80
1.06 × 102	0.85	0.68	2.47	2.66	1.11	4.80
1.06 × 101	3.38	6.97	1.76	1.42	1.99	1.61
1.06 × 100	3.61	9.06	2.12	2.89	0.17	2.05
1.06 × 10−1	1.54	9.87	-	-	2.22	3.12
1.06 × 10−2	-	1.94	-	-	0.24	1.25
1.06 × 10−3	-	-	-	-	-	-
	18S Leishmania spp.		kDNA L. donovani cplx
L. Donovani load	Intra Assay	Inter Assay	Intra Assay	Inter Assay
1.00 × 104	1.19	1.03	0.94	2.55
1.00 × 103	1.54	0.42	1.05	0.99
1.00 × 102	0.32	0.21	0.54	2.90
1.00 × 101	0.22	2.00	0.41	1.81
1.00 × 100	1.45	5.74	0.26	0.72
1.00 × 10−1	0.97	1.16	1.47	0.37
1.00 × 10−2	-	-	0.73	0.59
1.00 × 10−3	-	-	-	-