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. 2020 Mar 22;8(3):448. doi: 10.3390/microorganisms8030448

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Minimum spanning tree (MST) based on whole-genome multi-locus sequence typing (wgMLST) profiles of 162 presumptive ESBL-producing E. coli genomes along the One Health continuum from cattle feces, catch basins, surface streams, beef processing plants, municipal sewage, and humans: The MST includes a total of 9580 wgMLST loci and was constructed with BioNumerics (version 7.6.2). Each circle corresponds to a unique wgMLST profile and is colored based on sample origin. The size of the circle is proportional to the number of isolates sharing the same wgMLST profile, while the branch lengths correspond to the number of allele differences between isolates. Boxes highlight three major clusters of isolates of cattle and human origin.