Table 2.
Some walnut antioxidant peptides that have been identified.
| Hydrolase | SIT | ACS * | RMM orM/C | Ref |
|---|---|---|---|---|
| Pepsin | RP-HPLC-ESI-MS | ADAF | 423.23 Da | [19] |
| Trypsin | UPLC-ESI-MS / MS | WSREEQEREE | 1377 Da | [43] |
| Trypsin | UPLC-ESI-MS / MS | ADIYTEEAGR | 1124 Da | [43] |
| Neutral protease | RP-HPLC,nanoLC-ESI–MS/MS,de novo sequencing | LAGNPDDEFRPQ | 1357.6262 Da | [26] |
| Neutral Protease | RP-HPLC,nanoLC-ESI–MS/MS, de novo sequencing | VEDELVAVV | 971.5080 Da | [26] |
| Papain | RP-HPLC | CTLEW | 651.2795 Da | [47] |
| Tripsin, Viscozyme L | UPLC-ESI-Q-TOF-MS/MS | GGW | 319.1400 Da | [48] |
| Tripsin, Viscozyme L | UPLC-ESI-Q-TOF-MS/MS | VYY | 444.2129 Da | [48] |
| Tripsin, Viscozyme L | UPLC-ESI-Q-TOF-MS/MS | LLPF | 489.3071Da | [48] |
| Pancreatin | ESI-MS/MS | YS | 269.11 m/z | [12] |
| Pancreatin | ESI-MS/MS | YSVH | 505.23 m/z | [12] |
| Pancreatin | ESI-MS/MS | YK | 310.14 m/z | [12] |
| Pancreatin | ESI-MS/MS | YT | 283.13 m/z | [12] |
| Pancreatin | ESI-MS/MS | LPC | 331.16 m/z | [12] |
| Pancreatin | ESI-MS/MS | EM | 279.13 m/z | [12] |
| Pancreatin | ESI-MS/MS | CA | 192.14 m/z | [12] |
| Pancreatin | ESI-MS/MS | SQK | 362.3272 m/z | [12] |
| Pancreatin | ESI-MS/MS | CR | 288.2900 m/z | [12] |
| Pancreatin | ESI-MS/MS | CHC | 362.3265 m/z | [12] |
| Pancreatin | ESI-MS/MS | GHC | 316.3200 m/z | [12] |
| Pancreatin | ESI-MS/MS | YA | 253.1181 m/z | [12] |
| Pancreatin | ESI-MS/MS | YG | 239.1025 m/z | [12] |
| Pancreatin | ESI-MS/MS | NW | 318.3007 m/z | [12] |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | VEGNLQVLRPR | [44] | |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | LAGNPHQQQQ | [44] | |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | HNLDTQTESDV | [44] | |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | AGNDGFEYVTLK | [44] | |
| Alcalase protamex |
HPLC-FTMS, de novo sequencing, scoring method | WSVWEQELEDR | [44] | |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | QQRQQQGL | [44] | |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | AELQVVDHLGQTV | [44] | |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | EQEEEESTGRMK | [44] | |
| Alcalase and protamex | HPLC-FTMS, de novo sequencing, scoring method | VEDELVAVV | [44] | |
| Alcalase | LC-ESI-QTOF | AGGA | [42] | |
| Simulating gastrointestinal digestion | RP-HPLC-UPLC-QTOF-MS | TY | [21] | |
| Simulating gastrointestinal digestion | RP-HPLC-UPLC-QTOF-MS | SGGY | [21] |
The SIT, ACS, RMM, M/C andRefin the table refer to separation and identification technology, amino acid sequence, relative molecular mass, mass/charge ratio and reference respectively. * The amino acid sequence is in the form of 20 single letter abbreviations, namely G—Gly, A—Ala, V —Val, L—Leu, I—Ile, P—Pro, S—Ser, T—Thr, H—His, E—Glu, D—Asp, Q—Gln, N—Asn, K— Lys, R—Arg, C—Cys, M—Met, F—Phe, Y—Tyr and W—Trp.