Table 1.
Bacterial strains and plasmids in this study.
| Strains | Description | Reference |
|---|---|---|
| Escherichia coli | ||
| DH5α | F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ- | Invitrogen |
| BL21 (DE3) | F– ompT hsdSB (rB– mB–) gal dcm (DE3) | [18] |
| Plasmids | ||
| pET28a (+) | Expression vector with a hexahistidine tag, Kanr | Novagen |
| pETDuet-1 | Dual-expression vector with a hexahistidine tag, Ampr | Novagen |
| pM4LysΔTMD | pET28a-orf38ΔTMD1–206 | This study |
| M4Lys | pETDuet-1-orf38 | This study |
| ORF_37 | pETDuet-1-orf37 | This study |
| M4Lys-ORF_37 | pETDuet-1-orf37.orf38 | This study |
| M4LysΔTMD | pETDuet-1-orf38ΔTMD1–206 | This study |
| M4LysΔTMD-ORF_37 | pETDuet-1- orf38ΔTMD1–206.orf37 | This study |
| M4TMD | pETDuet-1-orf38TMD158–237 | This study |
| M4LysΔTMD-TMD | pETDuet-1- orf38ΔTMD1–206.TMD158–237 | This study |
| M4Lys1–226 | pET28a-orf381–226 | This study |
| M4Lys1–225 | pET28a-orf381–225 | This study |
| M4Lys1–224 | pET28a-orf381–224 | This study |