Table 2.

Primers used in this study.

Primer	Nucleotide Sequence [5′–3′] †	Restriction Site
BSPM4Lys-F-NdeI	ATG AGG AAA TAA CAT ATG GCT AAA CAG AAG	NdeI
BSPM4Lys-R-XhoI	GAA AAC GAG CGC CTC GAG GAC GCC CGT CTT	XhoI
BSPM4Lys2-R-XhoI	GGC CGC GCC GAA CTC GAG GCG TAC TCA ATC ACC	XhoI
BSPM4Lys5_3-R-XhoI	CTT CCG GTA CTC GAG TCA TCC CAT GTA CAG	XhoI
BSPM4Lys5_4-R-XhoI	CCG ATG CTT CTC GAG CGC TCA CCA TCC CAT	XhoI
BSPM4Lys6-R-XhoI	CCC GGC CCG ATG CTC GAG GTA CTA GAC CCA TCC	XhoI
BSPM4orf37-F-NcoI	CCC GCT AAT TTT TTG TGA GGA CCA TGG GC A TGA GCG AAA TGG AAC G	NcoI
BSPM4orf37-R-HindIII	GTT ATT GCG AAT CCC GCG AAG CTT CTG TTT AGC CAT CGG	HindIII
pETDuet-M4TMD-NcoI-F	CGA GGA AGG TCT GCC CAT GGG GGG CAT CGT TAA G	NcoI
pETDuet-M4TMD-HindIII-R	AAA ACG AGC GCC GCA AGC TTG CCC GTC TTG ATC C	HindIII
Duet-UP1-F	GAT GCG TCC GGC GTA GAG G	-
Duet-DOWN1-R	CGA TTA TGC GGC CGT GTA CAA T	-
Duet-UP2-F	ATT GTA CAC GGC CGC ATA ATC G	-
T7-promoter-F	TAATACGACTCACTATAGGG	-
T7-terminator-R	GCT AGT TAT TGC TCA GCG GTG	-

†, Restriction enzyme sites are underlined.