Figure 5.

(A) Effect of TO502-2403 (CSFCII) on the activity of RCs isolated at 14-h post-infection from MDBK cells that had been infected with wild-type BVDV (NADL) or with the selected TO502-2403^res BVDV strain. Reaction product of the RC assay was separated on a 1% agarose-glyoxal denaturing gel. RCs for these assays were either isolated from the MDBK cells infected with the wild-type virus or with the TO502-2403^res BVDV strain. (B) Densiometric analysis of the autoradiograph depicted in panel A and also from a second assay (picture not shown). Black bars represent the activity of the wild-type BVDV (NADL) RCs, and open bars represent the activity of RCs from the cell infected with TO502-2403^res virus. (C) Effect of TO505-6180 (CSFCI) on the activity of RCs isolated at 14-h post-infection from the MDBK cells that had been infected with wild-type BVDV (NADL) or with the selected TO505-6180^res BVDV strain. (D) Densiometric analysis of the autoradiograph depicted in panel C and also from a second assay (picture not shown). Black bars represent the activity of the wild-type BVDV (NADL) RCs, and open bars represent the activity of the RCs from the cell infected with the TO505-6180^res virus. Data are mean values ± SD of two independent experiments. UTC—untreated control; WT—wild-type.