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. 2019 Oct 18;26(3):204–214. doi: 10.1177/1753425919881778

Figure 5.

Figure 5.

(a) Bioinformatics analysis of matching sequence of miR‑26b within 3′‑UTR of CTGF. MuT CTGF 3′‑UTR is the mutation of the match sequence of 3′‑UTR of CTGF with miR‑26b (StarBase v.2.0). (b) Luciferase reporter assay revealed that miR‑26b binds to the 3′‑UTR of WT CTGF, not MUT CTGF. Relative luciferase activity was quantified, and the data were presented as mean ± SD. *P < 0.05 vs. respective NC groups. 3′‑UTR, 3′‑untranslated region; CTGF, connective tissue growth factor; miR, microRNA; NC, negative control; WT, wild type; MUT, mutant. (c) The predicted binding sites between PVT1 and miR-26b (DIANA TOOLS-LncBase v.2). (d) Luciferase activity was measured in human chondrocytes co-transfected with mimic NC or miR-26b mimic and PVT1-wt or PVT1-mut reporter at 48 h after transfection. PVT1 interacted directly with miR-26b (*P < 0.05, **P < 0.01).