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. 2020 Mar 2;10(4):1353–1360. doi: 10.1534/g3.120.401133

Figure 1.

Figure 1

Schematic of knock-down approach. A) Schematic representation of transgene present in transgenic line VgCp26.10. The transgene has an ECFP and DsRed marker genes. Three guide RNAs (sgECFP) target Cas9 to the ECFP gene. B) Representative fluorescence image of larvae showing parental wild-type, parental transgenic and exceptional Cyan-, DsRed+ phenotypes. C) Schematic comparison of embryo and adult injection approaches for ECFP knock-out and detection of mutants. The shaded box represents not done. Abbreviations are bp: base pair(s), pBac L/R: piggyBac left/right arm, SV40: simian virus 40 3′ untranslated region, DsRed: Discosoma species Red, ECFP: enhanced cyan fluorescent protein, sgECFP: single guide RNAs against ECFP, KO: knock-out, Gx: generation X.