Table 1. Injection data and comparison of ECFP mutation by adult and embryo injections.

Injection Mix			G-1				G0 Fluorescence Phenotype					G1 Fluorescence Phenotype
Protein	sgECFP	Saponin	G-1 Cross	No. Injected	Injection time PBM (hours)	No. on EC day	No.	Cyan+	Cyan-	Cyan-	Cyan Mosaic	G0 C+R+ Outcross	No.	Cyan+	Cyan-	Cyan-
								DsRed+	DsRed-	DsRed+	DsRed+			DsRed+	DsRed-	DsRed+
Adult injections: P2C-Cas9/gRNA mix injections into VgCp26.10 colony females (G-1) crossed with wild type males
0.5 µg/µL	0.75 µg/µL	+	VgCp26.10 Females X WT Males	300	24	212	425	106	317	2 (0.47%)	0	Female	295	103	192	0
												Male	622	324	298	0
0.75 µg/µL	1.2 µg/µL	+	VgCp26.10 Females X WT Males	83	48	60	194	160	24	10 (5.2%)	1* (1.2%)	Female	52	26	26	0
												Male	102	38	53	11 (10.8%)
0.75 µg/µL	1.2 µg/µL	+	VgCp26.10 Females X WT Males	63	47	33	161	52	107	2 (1.2%)	1** (0.62%)	Female	61	29	32	0
												Male	84	35	49	0
0.75 µg/µL	1.2 µg/µL	+	VgCp26.10 Females X WT Males	78	46	46	71	41	30	0	2** (2.8%)	Female	113	30	83	0
												Male	ND
			Sap+ Totals:	524		351	851	359	478	14 (1.7%)	4 (0.47%)		1329	585	733	11 (0.82%)
0.75 µg/µL	1.1 µg/µL	—	VgCp26.10 Females X WT Males	97	28	78	184	114	70	0	0	Female	217	96	118	3 (1.4%)
												Male	97	49	48	0
0.75 µg/µL	1.1 µg/µL	—	VgCp26.10 Females X WT Males	71	25	43	173	61	112	0	0	Female	41	15	26	0
												Male	207	109	98	0
			Sap - Totals:	168		121	357	175	182	0	0		562	269	290	3 (0.53%)
			Totals:	692		472	1208	534	660	0	0		1891	854	1023	14 (0.74%)
Embryo injections: Cas9/gRNA mix injections into homozygous VgCp26.10 transgenic embryos (G0)
0.2 µg/µL	0.075 µg/µL	—	VgCp26.10 Intercross				309	Not Screened					2320	2317	3 (0.13%)	0
0.2 µg/µL	0.3 µg/µL	—	VgCp26.10 Intercross				382	Not Screened					5646	5506	52 (0.92%)	88 (1.5%)
			Totals:				691						7966	7823	55 (0.69%)	88 (1.1%)

^*individual had one cyan+ eye and one cyan- eye.

^**these individuals had markedly reduced cyan fluorescence.