let-7-C expression inversely correlates with Br-Z3 and Chinmo expression in adult lineage clones during the larval-to-pupal transition. (A-C) Elav-Gal4,
UAS-mCD8::GFP-labeled lineage 8 clone stained for GFP (green in A-C), Br-Z3 (red A-C, white in A’-C’), and the let-7-Cp12.5kb::lacZ transcriptional reporter (blue in A-C, white in A”-C”). Single confocal sections from the apical to basal layers show late-to-early born neurons at pWL (A), P0 (B), and P24 (C). (D-F) Elav-Gal4, UAS-mCD8::GFP-labeled lineage 19 clone stained for GFP (green in D-F), Chinmo (blue in D-F, white in D’-F’), and the let-7-Cp12.5kb::lacZ transcriptional reporter (red in D-F, white in D”-F”). Single confocal sections from the apical to basal layers show late-to-early born neurons at pWL (D), P0 (E), and P24 (F). Scale bar: A-F, 15µm.