Figure 5.

Co-depletion of Br-Z3 and Chinmo leads to premature adult lineage elaboration. (A-L) Elav-Gal4, UAS-mCD8::GFP labeled UAS-Br-Z3-shRNA (A-B), chinmo¹ (C-D), and UAS-Br-Z3-shRNA; chinmo¹ (E-F), lineage 19 (A, C, E) and lineage 15 (B, D, F) clones stained for GFP (green). Insets in A show Br-Z3 expression in wildtype (top) and UAS-Br-Z3-shRNA (bottom) clones, indicating both the efficacy of the shRNA as well as the specificity of the antibody. Arrowheads indicate secondary outgrowths and are enlarged in insets at bottom. Clones were generated in newly hatched larvae and analyzed at WL. Scale bar: 15 μm.