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. 2020 Mar 16;25(6):1348. doi: 10.3390/molecules25061348

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Minimum inhibitory (MIC) and minimum bactericidal/fungicidal (MBC/MFC) concentrations of the SCGS estimation using a two-fold micro dilution method in 96 well micro-titer plates in comparison to a positive control (inoculated with microorganism without the SCGS) and a negative control (only sterile media). The plates were visually elaborated using a resazurin as an oxidation-reduction indicator at a concentration of 0.015%. The well with no color change (blue resazurin) means negative result or no growth however the changed color (pink color) means positive result or there is bacterial growth. (a, b) Were the plates of Staphylococcus aureus (DSMZ 3463), Bacillus subtilis (ATCC 6633) with more dilution. (c) The plate of Escherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027). (d) The plate of Candida albicans (ATCC 10231) and Aspergillus niger (ATCC 16404).