Figure 5.
Effect of M3G on the expression of MMP-1, -9 and type I procollagen in UVA-irradiated HaCaT keratinocytes. Cells were exposed to UVA radiation (10 J/cm2) and treated with given concentrations of M3G. After 24 h incubation, the mRNA expression of MMP-1 was analyzed with RT-PCR (upper panel) and RT-qPCR (lower panel) (A). RT-qPCR expression quantity was given relative to the untreated UVA-irradiated control, calculated by 2−(ΔΔCt) method. Protein levels of MMP-1, -9 and type I procollagen were determined by Western blotting (B). β-Actin was used as an internal control for RT-PCR, RT-qPCR and Western blotting. Retinoic acid was used as a positive control. Values are means ± SD of three independent experiment run in triplicate. * p < 0.05, ** p < 0.01 compared to UVA-irradiated untreated control.