Figure 7.
Effect of M3G on the expression of MMPs, type I procollagen and pro-inflammatory cytokines in UVA-irradiated HDFs. Cells were exposed to UVA radiation (10 J/cm2) and treated with given concentrations of M3G. After 24 h incubation cells were harvested for RT-qPCR and Western blotting. The mRNA expressions of MMP-1 and type I procollagen were determined by RT-qPCR and given as the relative expression quantity compared to untreated UVA-irradiated group (A). Protein levels of; MMP-1, -3, -9 and collagen (B) as well as pro-inflammatory mediators (COX-2, TNF-α, iNOS, IL-1β and IL-6) (C) were investigated by Western blotting. Values are means ± SD of three independent experiment run in triplicate. * p < 0.05, ** p < 0.01 compared to UVA-irradiated untreated control.