Table 2. Physicochemical Properties of MF NPs after Freeze-Drying Using Cryoprotectants at Different Concentrations (n = 3, mean ± s.d.).
| cryoprotectant | concentration (% w/v) | mean diameter (nm) | PDI | zeta potential (mV) | Sf/Sia |
|---|---|---|---|---|---|
| sucrose | 4% | 162 ± 12 | 0.44 ± 0.06 | –32 ± 1 | 1.38 |
| 6% | 206 ± 7.6 | 0.45 ± 0.02 | –39 ± 4 | 1.75 | |
| 8% | 146 ± 17 | 0.43 ± 0.02 | –30.4 ± 0.7 | 1.25 | |
| mannitol | 4% | 210 ± 31 | 0.39 ± 0.01 | –32.3 ± 2.6 | 1.78 |
| 6% | 256 ± 2.2 | 0.42 ± 0.01 | –36.5 ± 0.5 | 2.18 | |
| 8% | 206 ± 4.5 | 0.36 ± 0.02 | –31.3 ± 0.4 | 1.75 |
a
Sf/Si is the ratio of particle size after freeze-drying to particle size and before freeze-drying.