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(A) Western blots were performed to observe the PI3K, p-PI3K, AKT, p-AKT, mTOR, p-MTOR protein expression incubated with compound 12 (1 μM, 2 μM, 4 μM) for 48 h. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the control group. (B) The expression levels of LC3-II, Beclin-1, P62 and PI3K signalling were analysed by western blotting assay with or without pre-treatment of 3MA (500 μM). *p < 0.05, ***p < 0.001 compared with the control group.
