Figure 5.

EqPV-H can be transmitted by intra-articular (A) or intra-tendinous (B) injection. Allogeneic bone marrow-derived MSC were collected from a highly EqPV-H viremic horse. Cells were cultured in media containing either foetal bovine serum (MSC-FBS) or EqPV-H⁺ autologous horse serum (MSC-AHS), and washed twice in PBS before re-suspension in 1 ml PBS or EqPV-H⁺ AHS for inoculation. If horses did not become EqPV-H⁺ by 8 weeks, an additional inoculation with a higher viral load was administered, as indicated. SDFT, superficial digital flexor tendon; MSC, mesenchymal stromal cells; FBS, foetal bovine serum; PBS, phosphate-buffered saline; AHS, autologous horse serum; GE, genome equivalents.