Table 4.
Amino acid alterations of three conserved motifs of pbp1a, pbp2b, and pbp2x in 11 S. pneumoniae isolates.
| Strain no. | No. (%) of altered amino acids |
Changes in amino acids of conserved motifs forming or surrounding active pbp binding site |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
pbp1a 314–608 |
pbp2b 277–625 |
pbp2x 254–608 |
pbp1a | pbp2b | pbp2x | |||||||
| R6 | ─ | ─ | ─ | STMK | SRNVP | KTG | SVVK | SSNT | KTG | STMK | HSSN | LKSG |
| 20 | 1 (0.3) | 3 (0.9) | 1 (0.3) | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ |
| 36 | 1 (0.3) | 1 (0.3) | 0 (0.0) | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ |
| 46 | 1 (0.3) | 3 (0.9) | 2 (0.6) | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ |
| 69 | 16 (5.6) | 33 (9.5) | 32 (9.0) | ─ | ─ | ─ | ─ | ---A | ─ | ─ | L--- | V--- |
| 87 | 1 (0.3) | 3 (0.9) | 1 (0.3) | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ |
| 95 | 3 (1.0) | 3 (0.9) | 0 (0.0) | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ |
| 102 | 1 (0.3) | 1 (0.3) | 1 (0.3) | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ | ─ |
| 2a | 16 (5.6) | 29 (8.3) | 46 (13.0) | ─ | ─ | ─ | ─ | ---A | ─ | -A– | ─ | V--- |
| 3a | 40 (13.9) | 56 (16.1) | 40 (11.3) | -A– | ---T | ─ | ─ | ---A | ─ | -A– | ─ | V--- |
| 47a | 40 (13.9) | 18 (5.2) | 39 (11.2) | -A– | ---T | ─ | ─ | ---A | ─ | -A– | ─ | V--- |
aPenicillin-intermediate according to CLSI breakpoints.