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. 2020 Mar 14;9(1):571–581. doi: 10.1080/22221751.2020.1738278

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — MERTK binds to the CSFV E2 protein. (A and B) Gas6 and FBS have no effect in CSFV infection. Serum-starved PK-15 cells were incubated with CSFV-Rluc (MOI=0.01) in serum-free medium containing the indicated concentrations of pGas6 (A) or FBS (B). After 2 h, medium was replaced by medium supplemented with 4% FBS, and relative light units (RLU) were determined at 48 hpi. (C and D) MERTK interacts with the CSFV E2 protein. HEK293 T cells were co-transfected with pMERTK-Myc and pE2-Flag or pE^rns-Flag. At 48 hpt, the cells lysates were collected, and then subjected to Co-IP analysis using anti-Flag MAb (C) or anti-Myc MAb (D). (E) Affinity analysis of MERTK^ED-His and E2^ED-His. Surface plasmon resonance (SPR) assay characterizes the specific binding between the purified MERTK^ED-His and E2^ED-His proteins. (F) MERTK colocalizes with the E2 protein. The expression plasmids pMERTK-Myc and pE2-Flag were co-transfected into HEK293 T cells and subjected to confocal assay. Scale bar, 10 μm.