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. 2020 Mar 14;9(1):571–581. doi: 10.1080/22221751.2020.1738278

Figure 6.

Figure 6.

MERTK dampens innate immune response in CSFV infection of PK-15 cells. (A and B) LDC1267 reduced CSFV infection in a dose-dependent manner. PK-15 cells were pre-incubated with increasing concentrations of LDC1267 or DMSO for 30 min and were infected with CSFV-SM (MOI=0.01) in presence of the drug. At 48 hpi, the CSFV-SM genome copies (A) and progeny viral titers (B) were measured. The relative cell viability was determined by using CCK-8. (C and D) PK-15 cells were pre-incubated with LDC1267 (10 μM) or DMSO for 30 min, and cells were challenged with CSFV-SM (MOI=0.01) in the presence of the drug. Total cellular RNA was extracted at indicated time point, and relative viral RNA copies (C) and IFN-β mRNA level (D) were determined by RT-qPCR. (E) PK-15 cells transfected with siRNA targeting MERTK or negative control were infected with CSFV-SM at different MOIs (1 or 0.1). Total cellular RNA was extracted at 24 h, and relative viral RNA copies and IFN-β, SOCS1, GBP1, and OASL mRNA levels were determined by RT-qPCR. Means ± standard deviations (SD) of three technical replicates are shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001.