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. Author manuscript; available in PMC: 2020 Apr 9.
Published in final edited form as: J Neural Eng. 2019 Apr 10;16(4):046003. doi: 10.1088/1741-2552/ab1834

Figure 2.

Figure 2.

PatcherBot performance in cultured HEK cells. (a) Whole-cell success rate of pipettes over ten trials (nine reuses) in HEK cells. Reuses did not significantly decrease likelihood of whole-cell recording (odds ratio, OR = 1.17, CI: 0.13–1.18, P = 0.12). Numbers above columns indicate number of trials performed at each reuse number. (b) Seal resistances attained with and without cleaning. When cleaning is enabled (left), a pipette can achieve successive gigaseals (RGS ⩾ 1GΩ) on multiple cells. When cleaning is disabled (right) by draining Alconox from the clean bath, a gigaseal is obtained with a fresh pipette (0) but not on any other attempt (1–5). (c) Cumulative success rate in long-hold experiments. Increased unattended operation time contributed to slight diminishment in success rate (OR = 1.02, CI: 1.01–1.03, P = 0.0019, n = 74 attempts). Alconox was used as the cleaning agent.