Table 1.

Primers used for recloning into pET26b(+) and site-directed mutagenesis.

Enzymes	Sequence of primersa	Direction
Wild type	5′-GGAATTCCATATGCACTTCCCTAGAGCCTGTGTCTCCTCT-3′	Forward
	5′-ATCCGCTCGAGCGGTAAATGGCTCTGATACAAGCTGTG-3′	Reverse
H180A	5′-CTTTGTCTGGATGGCTTATTATGTGTCAA-3′	Forward
	5′-TTGACACATAATAAGCCATCCAGACAAAG-3′	Reverse
H202A	5′-CATTGATTTTGCCGCTGAAGCACCAGCTT-3′	Forward
	5′-AAGCTGGTGCTTCAGCGGCAAAATCAATG-3′	Reverse
H211A	5′-TTTTCTGCCTTGGGCTAGACTCTTCTTGT-3′	Forward
	5′-ACAAGAAGAGTCTAGCCCAAGGCAGAAAA-3′	Reverse
H363A	5′-TCTCAAAGCAGCATGGCCAATGCCTTGCACATC-3′	Forward
	5′-GATGTGCAAGGCATTGGCCATGCTGCTTTGAGA-3′	Reverse
H367A	5′-ATGCACAATGCCTTGGCCATCTATATGAATGGA-3′	Forward
	5′-TCCATTCATATAGATGGCCAAGGCATTGTGCAT-3′	Reverse
H389A	5′-CCTATCTTCCTTCTTGCCCATGCATTTGTTGAC-3′	Forward
	5′-GTCAACAAATGCATGGGCAAGAAGGAAGATAGG-3′	Reverse
H390A	5′-ATCTTCCTTCTTCACGCTGCATTTGTTGACAGT-3′	Forward
	5′-ACTGTCAACAAATGCAGCGTGAAGAAGGAAGAT-3′	Reverse

^aChanged bases are underlined.