Table 2.
Specific activity of the wild-type tyrosinase and mutant enzymes for l-tyrosine hydroxylation and l-dopa oxidation reactions.
| Enzyme | l-Tyrosine |
l-Dopa |
||
|---|---|---|---|---|
| Specific activity (μmol·μg−1·h−1) |
Relative activity (%) |
Specific activity (units/mg) |
Relative activity (%) |
|
| Wild type | 108.3 ± 2.1 | 100 | 1.53 ± 0.10 | 100 |
| H180A | 47.2 ± 1.2 | 44 | 1.35 ± 0.02 | 88 |
| H202A | 43.5 ± 0.9 | 40 | 1.17 ± 0.01 | 76 |
| H211A | 51.6 ± 0.8 | 48 | 1.37 ± 0.03 | 89 |
| H363A | 24.9 ± 0.3 | 23 | 0.23 ± 0.02 | 15 |
| H367A | 20.6 ± 0.3 | 19 | 0.34 ± 0.03 | 22 |
| H389A | 110.5 ± 0.2 | 102 | 1.04 ± 0.03 | 68 |
| H390A | 17.3 ± 0.5 | 16 | 0.40 ± 0.05 | 26 |
Values are means ± SD, generally based on n ≥ 5.