Table 2.

Photoswitching buffer [85].

Enzyme Stock Solution (A)
Ingredient	Concentration	Supplier and product number
10 µl catalase	20 µg/ml	Sigma, #C40
20 µl 1 M TCEP	4 mM	Sigma, #C4706
2.5 ml glycerol	50%	Fisher, #BP229-1
125 µl 1 M KCl	25 mM	Sigma, #P9333
100 µl 1 M Tris- HCl pH 7.5	20 mM	Sigma, #33742
5 mg glucose oxidase	1 mg/ml	Sigma, #G2133
Top up to 5 ml with distilled water and dispense into 50 µl aliquots and store frozen at −20 °C (for up to one year).

Glucose Stock Solution (B)
Ingredient	Concentration	supplier and product number
4 g glucose	100 mg/ml	Sigma, #G8270
4 ml glycerol	10%	Fisher, #BP229-1
Top up to 40 ml with distilled water and dispense into 400 µl aliquots and store at −20 °C (for up to one year).

Reducing Agent Stock Solution (C)
Ingredient	Concentration	supplier and product number
113.6 mg MEA-HCl	1 M	Sigma, #M6500
Top up to 1 ml with distilled water and store at 4 °C on the day of imaging. This solution can also be prepared in advance and stored at −20 °C for up to one year (do not refreeze).

Just prior to imaging the above solutions are mixed:

50 µl Solution A, 400 µl Solution B, 100 µl Solution C, 450 µl PBS.