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Time-of-addition assay. 120 μM W-9, or 160 μM W-13, W-15, or ribavirin, were added to Hep-2 cells for different time periods after CVB3 infection. Progeny virus yield was determined at 12 h post-infection ((−1)–0 h, viral infection period; 0–10 h, period of virus proliferation in cells). Values are presented as means ± SDs. * p < 0.05; ** p < 0.01, compared with the virus control group.
