Figure 5.

Effect of W13 on CVB3 replication in Hep-2 cells. Hep-2 cells infected with 100 TCID₅₀ CVB3 were incubated in the absence or presence of 160 µM W13 and harvested at the indicated times post-infection. (a) Progeny virus yields were determined. (b) Viral RNA levels were measured by analysis of total RNA. GAPDH was amplified and the resulting data was used for normalization. Values represent the means ± SDs of three independent experiments. * p < 0.05; ** p < 0.01, compared with the virus control group. (c) CVB3 protein levels were determined by indirect immunofluorescence, using the mouse anti-coxsackie virus B3 monoclonal antibody, MAB948, and an alexa-fluor-488-conjugated affiniPure goat anti-mouse IgG (H + L), after incubation with W13 for 24 h. Nuclei were stained with DAPI and green foci indicate the presence of CVB3 protein.