Table 3.
Examining the opioid receptor mediation in G-protein activity ([35S]GTPγS specific binding normalized to basal activity) of 14-OMeC6SU in the presence or absence of 10 µM naloxone in [35S]GTPγS binding assays performed in rat and guinea pig brain membrane homogenates. 14-OMeC6SU was added in 10 and 100 µM in rat and guinea pig brain membranes, respectively.
| [35S]GTPγS Specific Binding ± S.E.M. (%) |
||
|---|---|---|
| Compounds | Rat Brain | Guinea Pig Brain |
| 14-OMeC6SU | 130.1 ± 7.99 (n = 7) |
128.7 ± 2.08 (n = 5) |
| +10 µM naloxone | 96.24 ± 4.51 ** (n = 5) |
95.84 ± 1.34 *** (n = 5) |
* compared to C6SU (unpaired t test, two-tailed P-value, **: P < 0.01; ***P < 0.001).