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. 2020 Jan 9;217(4):e20190930. doi: 10.1084/jem.20190930

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© 2020 Liu et al.

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Figure S5. — Epac1 deletion in astrocytes does not affect RGC death after IR. (A) GFAP-Cre mice were crossed with Rosa26YFP reporter mice to generate GFAP-Cre; Rosa26YFP mice. Retinal flatmounts were stained with antibodies against YFP (green) and astrocyte marker GFAP (red). (B) OCT analysis in live Epac1^fl/fl and GFAP-Cre; Epac1^fl/fl mice for retinal thickness 7 d after IR. Yellow H lines indicate the thickness of GCC. Bar graph represents the thickness of GCC. n = 5–7 mice. Scale bar: 100 µm. (C) Representative images of retinal flatmounts labeled with Tuj1 antibody (green) in Epac1^fl/fl and GFAP-Cre; Epac1^fl/fl mice 7 d after IR. Scale bar: 50 µm. Bar graph represents the number of Tuj1-positive cells per field. n = 6–7 mice; eight images were taken at the peripheral retina for each sample and calculated as average value. ****, P < 0.0001; one-way ANOVA. Error bars represent SEM.