Figure 4.

T cell–intrinsic caspase-1 is required for host protection against Cr. (A) Schematic of the experiment. (B) Representative intracellular cytokine staining of IFNγ, IL-22, and IL-17A in CD4⁺CD90⁺CD44⁺ T cells in the mLNs at 10 dpi of Cr. (C) Quantification of IL-17A⁺IL-22⁻, IL-17A⁺IL-22⁺, IL-17A⁻IL-22⁺ percentages (left) and IFNγ⁺IL-17A⁺%, IFNγ⁺IL-17A⁻ percentages (right). n = 7–8 mice per group. (D) Cr burden (quantified as CFU) in the cecum at 10 dpi (left). Also shown is the Cr burden when normalized to cecum content weight (right). NT, nontransferred (i.v. PBS). n = 4–9 mice per group. (E) Representative image of stool appearance (left) and quantified stool water content (right, as percentage of water in each pellet). n = 4–7 mice per group. Data are representative of or combined from two independent experiments. Each data point represents one biological replicate. Statistics represent mean ± SEM, and P values were determined by unpaired Student’s t test (C) or one-way ANOVA (D and E). ns, not significant; *, P < 0.05; **, P < 0.01.