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. 2020 Jan 17;217(4):e20191869. doi: 10.1084/jem.20191869

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© 2020 Etzerodt et al.

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Figure 2. — Single-cell transcriptional profiling of omental macrophages after tumor colonization. (A) Whole-mount confocal imaging of omentum showing the location of FALCs by visualizing collagen IV (ColIV, green), adipocytes (LipidTox, red), and leukocyte clusters (CD45, white); scale bar: 0.5 mm. (B) Whole-mount imaging of ID8-Luc infiltration in the omentum 24 h after injection of ID8 cells. Pecam-1 staining for blood vessels (red), CD163 for macrophages (green), CD45 for FALCs (white), and Qdot⁷⁰⁵ for ID8 cells (magenta); scale bar: 100 µm and 50 µm in magnification. (C) Two-dimensional representation and clustering of single-cell TAM transcriptomes using UMAP (n = 16,514). (D) Heatmap showing the top 10 DEGs in each UMAP cluster. (E) Violin plots showing expression of commonly used macrophage markers.