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. 2020 Jan 17;217(4):e20191869. doi: 10.1084/jem.20191869

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© 2020 Etzerodt et al.

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Figure S2. — Fate mapping of omental macrophages. (A and B) Flow cytometric analysis of blood monocytes in mice 5 wk (A) or 13 wk (B) after adoptive transfer of CD45.1⁺/CD45.2⁺ donor bone marrow in irradiated CD45.1⁺ host mice that were protected by a lead shield. Monocytes were gated as Live (FSC-A versus Sytox Blue), CD5^neg, CD19^neg (CD5 versus CD19), NK1.1^neg, CD11b⁺ (CD11b versus NK1.1), and Ly6C⁺, Ly6G^neg (Ly6C versus Ly6G). Data are represented as mean ± SEM of n = 5. (C) Flow cytometric analysis of YFP⁺ microglia in Cx3Cr1CreER:R26-yfp mice pulse-labeled with tamoxifen at E16.5. Microglia were gated as Live (FSC-A versus Sytox Blue), Ly6C^neg, CD11c^neg (Ly6C versus CD11c), and CD45.2^dim, CD11b^hi (CD11b versus CD45.2). Data are represented as mean ± SEM of n = 6. All data are representative of two independent experiments.