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. 2020 Jan 22;217(4):e20191374. doi: 10.1084/jem.20191374

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© 2020 Reed-Geaghan et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 2. — Labeling of peripheral monocyte populations following tamoxifen exposure. (A) Representative flow sorting scheme for tdTomato expression in Ly6C^hi and Ly6C^lo monocytes from the spleen. FSC, forward scatter; SSC, side scatter. (B and C) Fraction of Ly6C^hi and Ly6C^lo monocytes in the blood (B) or spleen (C) expressing tdTomato in Cx3cr1^CreERT2; ROSA26^tdTomato mice at 2, 4, or 8 mo of age following tamoxifen or control injections at 1 mo of age. Data presented as mean ± SEM from five or more mice/age/treatment from three or more independent experiments. (D) Fraction of Ly6C^hi and Ly6C^lo monocytes expressing tdTomato in Ccr2^CreERT2; ROSA26^tdTomato mice. Data presented as mean ± SEM from ≥15 mice/age/treatment from ≥3 independent experiments. TMX, tamoxifen. (E) Representative confocal images of IHC for Iba1 and tdTomato in the choroid plexus and on the brain surface in 8-mo-old Ccr2^CreERT2; 5xFAD; ROSA26^tdTomato mice treated with tamoxifen for 28 d. Scale bar, 15 µm.