Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 30;217(4):e20182091. doi: 10.1084/jem.20182091

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Tang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S1. — Silencing the CBLB gene in human macrophages leads to heightened IL-1β and TNF-α production upon LPS priming and stimulation with ATP, CTB, and EHEC. Related to Fig. 1. (A) ELISA analysis of IL-1β and TNF-α production by human MDMs transfected with control siRNA or CBLB-specific siRNA (200 nM) by using Lonza nucleofector reagent before priming with LPS (100 ng/ml) and stimulation with ATP (2.5 nM, 30 min), CTB (20 ng/ml, 6 h), and EHEC (MOI = 25:1, 8 h). Data are shown as mean ± SD. **, P < 0.01; Student’s t test. (B) Immunoblot analysis of CBLB in MDMs treated with control siRNA or CBLB-specific siRNA. Actin was used as a loading control. Data are representative of three independent experiments.